An important prospective use of our AS-PCR design tool is rapid testing for specific variations in pathogen genomes. For example, the four-way genotyping assay may provide information on …

Standard PCR reagents include a set of appropriate primers for the desired target gene or DNA segment to be amplified, DNA polymerase, a buffer for the specific DNA polymerase, …

The results of this study demonstrate that incorporating an IC into PCR-based tests increases sensitivity by enabling the user to identify and retest samples inhibitory to PCR.

Jul 7, 2025 · Kary Mullis introduced PCR in 1985 and was later granted the Nobel Prize in Chemistry for this contribution. PCR enables precise detection and analysis of amplified DNA …

Abstract Primers are critical components of any PCR assay, as they are the main determinants of its specificity, sensitivity, and robustness.

Through careful primer and PCR design, differences in splice isoform expression in cDNA generated from total RNA can be visualized using agarose gel electrophoresis.

We provide a concise overview of the main primer-related issues that confront anyone wanting to design a qPCR assay, consider the main criteria that have an impact on assay performance, …

It addresses the basic fluorescent chemistries of real-time PCR, the basic rules and pitfalls of primer design, and provides a step-by-step protocol for designing SYBR® Green-based …

Abstract While conventional PCR applications typically focus on a single PCR assay per reaction, multiplex PCR applications are a convenient and scalable solution becoming more routine.

Multiplex polymerase chain reaction (PCR) has multiple applications in molecular biology, including developing new targeted next-generation sequencing (NGS) panels. We present …