The internet is filled with different methods, procedures, and workflows that can overwhelm you during data analysis, especially if this is your first time with RT-qPCR. This article is going to …

Jul 3, 2024 · The article will show you two methods on how to analyze qPCR data in Excel. Download our practice workbook and follow us.

In this section, general guidelines and a sample gene expression experiment are presented to demonstrate how to perform gene expression analysis using real-time PCR.

Jun 17, 2023 · There are two main ways to analyze qPCR data: double delta Ct analysis and the relative standard curve method (Pfaffl method). Both methods make assumptions and have …

This document introduces statistical analysis of qPCR data in Qlucore Omics Explorer (QOE). It is assumed that the user is familiar with the basic functionality of QOE.

In this detailed tutorial, we walk you through a complete step-by-step analysis of qPCR data, including selecting the best reference genes, applying the ΔCq method, and using the …

Furthermore, the absence of raw data and analysis code limits the community's ability to evaluate potential biases and reproduce findings. Here, we primarily aim to encourage researchers to …

This guide will walk you through every step of analyzing your qPCR data using the widely accepted ΔΔCt method, from organizing your raw data to creating publication-ready graphs.

After a traditional PCR has been completed, the data are analyzed by resolution through an agarose gel or, more recently, through a capillary electrophoresis system. For some …

Jul 2, 2025 · Analyzing RT-PCR data accurately is crucial for deriving meaningful biological insights. This article delves into the essential steps and considerations for analyzing RT-PCR …